Activities at the CeMI

Four Principal Investigators (PIs) from the Institute for Integrated Cell-Material Sciences (iCeMS), Yoshie Harada (Director of the CeMI), John Heuser, Aki Kusumi, and Koichiro Tanaka, as well as the Deputy Director of the CeMI, Takahiro Fujiwara (Senior Lecturer of the iCeMS), form the core group of the CeMI. In addition, iCeMS Kyoto Fellows (also PIs), Pete Carlton and Ziya Kalay, and the Visiting Professor Fumiyoshi Ishidate, participate in the CeMI.

We aim (1) to develop new, powerful technologies for imaging the restless nano- to meso-scale universe of biomolecular complexes in living cells, at the spatiotemporal resolutions of functioning single molecules, and (2) to make these technologies available quickly to the worldwide scientific community, for the further advancement of cellular meso-science.

The Center has the following four specific areas of activity:

1. Core Research

Technology development and initial applications, which are conducted in the laboratories of the core PIs as well as in the CeMI.

2. Collaborative Research

 Following the development of new, pilot technologies and instruments by CeMI’s core members, the first instruments for practical applications will be installed at the CeMI and then made available to all interested parties on a collaborative research basis. New, broad applications are expected to lead to further groundbreaking technologies and instruments. In addition, selected new technologies developed elsewhere will be implemented at the CeMI, to incorporate important advances as soon as possible.

The following CeMI-built stations are currently in operation:

1-. Four, single fluorescent-molecule tracking (SFMT) stations, each with various specific capabilities, including simultaneous three-color SFMT (unique in the world; see photo), photoactivation, and the world’s fastest frame-rate at 10 kHz (all operable for live cells at 37 ˚C in a 5% CO2 atmosphere).

2-. Two terahertz microscopes with the world’s fastest image acquisition rate (10 Hz).

Other advanced, commercial confocal/time-lapse fluorescence microscopes are also available.

3.Education and Training

The center will hold symposia, seminars, workshops, and hands-on training sessions, open to the global scientific community.

4. Services

Commercial instruments, including those with both standard and advanced capabilities, are available to the iCeMS scientists as well as to researchers outside of the iCeMS wishing to use the instruments for collaborative studies with iCeMS scientists. Refer to the "Rules for Use" for access details.

The microscopes currently available for use include a confocal (multiphoton) fluorescent microscope, a time-lapse fluorescent microscope, a STED confocal fluorescent microscope and others. Refer to the "List of Equipment" for details.

On a limited basis, CeMI personnel are available to obtain data for interested users, but only when the users are physically present.

The CeMI’s aim is to become a world hub, where scientists from around the globe can gather to engage in meso-bio, single-molecule imaging, to develop cellular meso-science. For details of any of the services provided, please contact below.

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