CeMIの設備一覧
現在、CeMIでは下記の表の通り様々な設備をそろえております。
初めて予約される方は、＜ご利用案内＞ をお読み下さい。
CeMI設備の使用許可をお持ちの方は、＜オンライン予約ページ＞ へお進み下さい。ログインIDには、@cemi.icems.kyoto-u.ac.jpをつけてください。
【 表の見方：場所について 】
*「RB2」 = iCeMS 総合研究1号館 2F
*「RA3」 = iCeMS 総合研究1号館 別館 3F
*「IR3」 = iCeMS研究棟 ３F
【 設備配置図 (pdfファイル) 】
総合研究1号館 2F および 総合研究1号館 別館 3F
総合研究1号館 2F （拡大図）
総合研究1号館 別館 3F （拡大図）
| No. | 名称 | 略称 | 場所 |
ニコン・イメージングテクノラボ |
|||
| 1. | ニコン 高速共焦点倒立顕微鏡 A1R | Nikon FastConf | RB2-209 |
| 2. | ニコン 汎用共焦点倒立顕微鏡 A1 | Nikon Conf | IR3-309 |
| 3. | ニコン 長期タイムラプス顕微鏡（バイオステーション） | Nikon BioStation | RB2-209 |
| 4. | ニコン 全反射顕微鏡（光ファイバー型） | Nikon FiberTIRF | RB2-203 |
| 5. | １分子追跡ステーション（高速） | SFMT-HS | RB2-203 |
| 6. | １分子追跡ステーション（ベンチテスト機） | SFMT-Bench | RB2-203 |
| 7. | １分子追跡ステーション（生細胞PALM開発機） | SFMT-PALM-Dev | RB2-209 |
オリンパス・マイクロイメージングセンター |
|||
| 1. | オリンパス 共焦点倒立顕微鏡 FV1000-Inv | Olympus InvConf | RA3-305 |
| 2. | オリンパス 共焦点正立顕微鏡 FV1000-UR | Olympus URConf | RA3-305 |
| 3. | オリンパス 生細胞観察用タイムラプス倒立蛍光顕微鏡 | Olympus Time-Lapse | RA3-305 |
| 4. | オリンパス 全反射顕微鏡（光ファイバー型） | Olympus FiberTIRF | RA3-305 |
| 5. | １分子追跡ステーション（開発用機） | SFMT-Dev | RA3-304 |
ライカセンター |
|||
| 1. | ライカ STED共焦点・FCS共焦点顕微鏡 | Leica STEDConf | RA3-301 |
ツァイスセンター |
|||
| 1. | ツァイス 2光子励起・共焦点・FCCS顕微鏡 (LSM780) | Zeiss 2pConf | RA3-301 |
| 2. | ツァイス Airyscan超解像・共焦点顕微鏡 (LSM880) | Zeiss LSM880Airy | RA3-301 |
| 3. | ツァイス 共焦点顕微鏡 (LSM710) | Zeiss Conf | RA3-304 |
テラヘルツ・イメジングセンター |
|||
| 1. | 実時間テラヘルツ近接場顕微鏡 | THz-NF | RB2-201 |
| 2. | 実時間テラヘルツ透過顕微鏡 | THz-TR | RB2-201 |
CeMIの設備による成果リスト
Nikon FastConf (A1R)
Suzuki KGN, Kasai RS, Hirosawa KM, Nemoto YL, Ishibashi M, Miwa Y, Fujiwara TK, Kusumi A (2012) Transient GPI-anchored protein homodimers are units for raft organization and function. Nat Chem Biol 8: 774-783
DOI: 10.1038/Nchembio.1028
Inaba H, Sanghamitra NJM, Fukai T, Matsumoto T, Nishijo K, Kanamaru S, Arisaka F, Kitagawa S, Ueno T (2014) Intracellular protein delivery system with protein needle-GFP construct. Chem Lett 43: 1505-1507
DOI: 10.1246/cl.140481
Sanghamitra NJM, Inaba H, Arisaka F, Ohtan Wang D, Kanamaru S, Kitagawa S, Ueno T (2014) Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif. Mol BioSyst 10: 2677-2683
DOI: 10.1039/C4MB00293H
Inaba H, Sanghamitra NJ, Fujita K, Sho T, Kuchimaru T, Kitagawa S, Kizaka-Kondoh S, Ueno T (2015) A metal carbonyl-protein needle composite designed for intracellular CO delivery to modulate NF-kB activity. Mol Biosyst 11: 3111-3118 DOI: 10.1039/C5MB00327J
Nikon FiberTIRF
Kafer GR, Li X, Horii T, Suetake I, Tajima S, Hatada I, Carlton PM (2016) 5-Hydroxymethylcytosine marks sites of DNA damage and promotes genome stability. Cell Rep 14: 1283-1292
DOI: 10.1016/j.celrep.2016.01.035
Nikon SFMT-HS
Shibata ACE, Fujiwara TK, Chen LM, Suzuki KGN, Ishikawa Y, Nemoto YL, Miwa Y, Kalay Z, Chadda R, Naruse K, Kusumi A (2012) Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone. Cytoskeleton 69: 380-392
DOI: 10.1002/Cm.21032
Shibata ACE, Chen LMH, Nagai R, Ishidate F, Chadda R, Miwa Y, Naruse K, Shirai YM, Fujiwara TK, Kusumi A (2013) Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis. Cytoskeleton 70: 161-177
DOI: 10.1002/Cm.21097
Nikon SFMT-PALM-DEV
Fujiwara TK, Iwasawa K, Kalay Z, Tsunoyama TA, Watanabe Y, Umemura YM, Murakoshi H, Suzuki KGN, Nemoto YL, Morone N, Kusumi A (2016) Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane. Mol Biol Cell 27: 1101-1119
DOI: 10.1091/mbc.E15-04-0186
Sotoma S, Iimura J, Igarashi R, Hirosawa KM, Ohnishi H, Mizukami S, Kikuchi K, Fujiwara TK, Shirakawa M, Tochio M (2016) Selective labeling of proteins on living cell membranes using fluorescent nanodiamond probes. Nanomaterials 6: 56
DOI: 10.3390/nano6040056
Olympus InvConf
Komura N, Suzuki KGN, Ando H, Konishi M, Ikeda M, Imamura A, Chadda R, Fujiwara TK, Tsuboi H, Sheng R, Cho W, Furukawa Koichi, Furukawa Keiko, Yamauchi Y, Ishida H, Kusumi A (2016) Raft-based interactions of gangliosides with a GPI-anchored receptor.
Nat Chem Biol 12:402-410
DOI:10.1038/nchembio.2059
Olympus InvConf & URConf (FV1000D), Nikon FastConf (A1R), and Leica STEDConf (SP5 STED)
Sugimura K, Ishihara S (2013) The mechanical anisotropy in a tissue promotes ordering in hexagonal cell packing. Development 140: 4091-4101
DOI: 10.1242/Dev.094060
Olympus InvConf & URConf (FV1000D)
Ishihara S, Sugimura K (2012) Bayesian inference of force dynamics during morphogenesis. J Theor Biol 313: 201-211
DOI: 10.1016/j.jtbi.2012.08.017
Ishihara S, Sugimura K, Cox SJ, Bonnet I, Bellaiche Y, Graner F (2013) Comparative study of non-invasive force and stress inference methods in tissue. Eur Phys J E Soft Matter 36: 9859 Cover page , DOI: 10.1140/Epje/I2013-13045-8
Olympus InvConf & Zeiss 2pConf (LSM780 NLO/ConfoCor3)
Oomoto I, Suzuki-Hirano A, Umeshima H, Han YW, Yanagisawa H, Carlton P, Harada Y, Kengaku M, Okamoto A, Shimogori T, Wang, DO (2015) ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues. Nucleic Acids Res 43: e126
DOI: 10.1093/nar/gkv614
Zeiss 2pConf (LSM780 NLO/ConfoCor3)
Diring S, Wang DO, Kim C, Kondo M, Chen Y, Kitagawa S, Kamei K, Furukawa S (2013) Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform. Nat Commun 4: 2684
DOI: 10.1038/Ncomms3684
Takamatsu S, Onoguchi K, Onomoto K, Narita R, Takahasi K, Ishidate F, Fujiwara TK, Yoneyama M, Kato H, Fujita T (2013) Functional characterization of domains of IPS-1 using an inducible oligomerization system. PLoS One 8: e53578
DOI: 10.1371/journal.pone.0053578
Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R (2013) Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science 342: 1203-1208
DOI: 10.1126/science.1242366
Yoshii T, Ikeda M, Hamachi I (2014) Two-photon-responsive supramolecular hydrogel for controlling materials motion in micrometer space. Angew Chem 126: 7392-7395
DOI: 10.1002/ange.201404158
Sadaie W, Harada Y, Matsuda M, Aoki K (2014) Quantitative in vivo FCCS analyses highlight the importance of competitive effects in the regulation of protein-protein interactions. Mol Cell Biol 34: 3272-3290
DOI: 10.1128/MCB.00087-14
Nakatsuji H, Numata T, Morone N, Kaneko S, Mori Y, Imahori H, Murakami T (2015) Thermosensitive ion channel activation in single neuronal cells by using surface-engineered plasmonic nanoparticles. Angew Chem Int Edit 54: 11725-11729
DOI: 10.1002/anie.201505534
Oh SW, Onomoto K, Wakimoto M, Onoguchi K, Ishidate F, Fujiwara T, Yoneyama M, Kato H, Fujita T (2016) Leader-containing uncapped viral transcript activates RIG-I in antiviral stress granules. PLoS Pathog 12: e1005444
DOI: 10.1371/journal.ppat.1005444
Takano Y, Numata T, Fujishima K, Miyake K, Nakao K, Grove WD, Inoue R, Kengaku M, Sakaki S, Mori Y, Murakami T, Imahori H (2016) Optical control of neuronal firing via photoinduced electron transfer in donor–acceptor conjugates. Chem Sci 7: 3331-3337
DOI: 10.1039/C5SC04135J
Hamamatsu EMCCD camera (ImagEM)
Yoshikawa N, Hirori H, Watanabe H, Aoki T, Ihara T, Kusuda R, Wolpert C, Fujiwara TK, Kusumi A, Kanemitsu Y, Tanaka K (2013) Biexciton state causes photoluminescence fluctuations in CdSe/ZnS core/shell quantum dots at high photoexcitation densities. Phys Rev B 88: 155440
DOI: 10.1103/Physrevb.88.155440
Tracking and data analysis software developed at the CeMI
Tanaka KA, Suzuki KG, Shirai YM, Shibutani ST, Miyahara MS, Tsuboi H, Yahara M, Yoshimura A, Mayor S, Fujiwara TK, Kusumi A (2010) Membrane molecules mobile even after chemical fixation. Nat Methods 7: 865-866
DOI: 10.1038/nmeth.f.314
Kasai RS, Suzuki KG, Prossnitz ER, Koyama-Honda I, Nakada C, Fujiwara TK, Kusumi A (2011) Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging. J Cell Biol 192: 463-480
DOI: 10.1083/jcb.201009128
Koyama-Honda I, Itakura E, Fujiwara TK, Mizushima N (2013) Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9: 1491-1499
DOI: 10.4161/auto.25529
Nishimura H, Ritchie K, Kasai RS, Goto M, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, Kusumi A (2013) Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging. J Cell Biol 202: 967-983
DOI: 10.1083/jcb.201301053
Hiramoto-Yamaki N, Tanaka KA, Suzuki KG, Hirosawa KM, Miyahara MS, Kalay Z, Tanaka K, Kasai RS, Kusumi A, Fujiwara TK (2014) Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes. Traffic 15: 583-612
DOI: 10.1111/tra.12163